CDS target predictions are based on CDS datasets from Ensembl or JGI Databases.

  Degradome identification of CDS targets are based on published degradome datasets deposited in NCBI.

  Intergenic target predictions are based on Ensembl genomic sequences. Intergenic sequences are retrieved according to Ensembl genomic annotations (miRNA precursor sequences are masked to avoid being captured as targets).

  Intergenic eTM predictions are based on Ensembl intergenic sequences.

  Noncanonical target predictions are based on modified CleaveLand analysis of degradome datasets.

 

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FAQs

1. What's the criteria used in the degradome analysis?

Our degradome analysis was performed using CleaveLand4, based on (i) degradome category 0 or 1; (ii) Allen et al reported score <5.

2. What's the non-canonical case I and II?

Both case I and case II non-canonical sites have central (p9-p11) mismatch/bulge. Case I cleavage occurs between alignment positions 10 and 11, while case II cleavage site slightly shifts (between p9-p10 or p11-p12) on the target strand.

 

 

 

Copyright @ 2016 Contact: Dr. Xuan Ma
College of Life Sciences,Tianjin Normal University,Tianjin, 300387, China