TarDB: A miRNA Target Database in Plants.


TarDB employs cross-species conservation filter, degradome and sRNA-seq data to identify high-confidence miRNA targets in plants.



miRNA ID

ath-miR774b-5p

Organism

Arabidopsis thaliana

Species

Mature miRNA Sequence(5'-3')

UGAGAUGAAGAUAUGGGUGAU

miRBase

Precursor miRNA Sequence(5'-3')

GAGUGUUGAUUAUAUGGAUUCAUUUGAGAUGAAGAUAUGGGUGAUGAUCAAUGCUUACGAUUUUGGUCAAUUUAUGUUAGCAAUUUAAUUCUUUUUCAUCCAUAUUUUCAUCUCGAAUUUAAUCUUUAAAAAAGCGUUU

miRBase

Precursor miRNA Structure(5'-3') 

GAGU   GAUUAUAU     -C                       --------------------------       UGCU 
    GUU        GGAUU  AUUUGAGAUGAAGAUAUGGGUGA                          UGAUCAA    U
    |||        |||||  |||||||||||||||||||||||                          |||||||    A
    CGA        UCUAA  UAAGCUCUACUUUUAUACCUACU                          ACUGGUU    A
UUUG   -AAAAAUU     UU                       UUUUCUUAAUUUAACGAUUGUAUUUA       UUAG

Homologous Mature miRNAs   miRNAs are clustered by CD-HIT with sequence identity of >85% and word size of 5.

aly-miR774a-5p.1      CGAGACGAAGAUAUGGGUGAC
ath-miR774b-5p        UGAGAUGAAGAUAUGGGUGAU
                       **** **************