SCORING: Mismatches or Indels are penalized 1 score. G-U wobbles are penalized 0.5 score. Penalties at positions 2-12 from miRNA 5' end are doubled. If positions 3-5 have mismatches, additional 1 score is penalized.

Total mispairs include all mismatches and Indels (insertions and deletions)

Seed region includes positions 2-7 from miRNA 5' end.

In most cases, miRNA mediated cleavage occurs when positions 9-11 have perfect match.

Cat.0, Cat.1: max. sequencing peak; Cat.2: above average sequencing depth

Allen et al. score: mismatches or bulges are penalized 1. G-U wobbles are penalized 0.5. The penalties at positions 2-13 are doubled.

CleaveLand P-value calculates the binomial probability of observations in a number of trials.

Total mispairs include all mismatches and Indels (insertions and deletions)